A Sensitive Radioassay for Sulfhydryl Groups with Tetraethylthiuram Disulfide
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چکیده
منابع مشابه
Interaction between tetraethylthiuram disulfide and the sulfhydryl groups of D-amino acid oxidase and of hemoglobin.
Inhibition of the crystallized hog kidney flavoenzyme, D-amino acid oxidase, by tetraethylthiuram disulfide (disulCram, TETD) has been found to involve the sulfhydryldisulfide exchange reaction; primary products of the interaction between oxidase and reagent are diethyldithiocarbamate ion and a derived protein carrying mixed disulfide linkages, each of which consists of an enzyme thiol sulfur a...
متن کاملSulfhydryl and Disulfide Groups of Proteins
1. Methods have been described for reducing protein S-S groups, for oxidizing protein SH groups, and for estimating protein S-S and SH groups. 2. It has been found necessary in estimating the cystine content of proteins by the Folin-Marenzi method to take into account any cysteine that may be present. 3. A method for estimating the cysteine content of proteins has been described. 4. With these ...
متن کاملBioassay of tetraethylthiuram disulfide for possible carcinogenicity.
A bioassay of technical-grade tetraethylthiuram disulfide for possible carcinogenicity was conducted by administering the test chemical in feed to F344 rats and B6C3F1 mice. Groups of 50 rats of each sex were administered tetraethylthiuram disulfide in the diet at one of two doses, either 300 or 600 ppm, for 107 weeks. Groups of 50 mice of each sex were administered the test chemical at one of ...
متن کاملAntithyroid action of tetraethylthiuram disulfide.
Tetraethylthiuram disulfide (Antabuse) has been demonstrated to be clinically useful in treating alcoholism (1). The compound has been shown to inhibit the oxidation of acetaldehyde (2) and, further, to inhibit the action of rat liver xanthine oxidase to the extent of 71 per cent, and the succinoxidase system 100 per cent (3). Examination of the chemical structure of Antabuse indicates that it ...
متن کاملDifferential analysis of sulfhydryl and disulfide groups of intact spores.
Blankenship, L. C. (Eastern Utilization Research and Development Division, Washington D.C.), and M. J. Pallansch. Differential analysis of sulfhydryl and disulfide groups of intact spores. J. Bacteriol. 92:1615-1617. 1966.-Fluorescence quenching of fluorescein mercuric acetate in alkaline medium (1 n NaOH) was found to be an accurate, sensitive method for differential analysis of sulfhydryl and...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1966
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)96493-8